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cag gfp  (Vector Biolabs)


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    Vector Biolabs cag gfp
    Cag Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+dj+cag+gfp/pm39028743-53-1-9?v=Vector+Biolabs
    Average 95 stars, based on 9 article reviews
    cag gfp - by Bioz Stars, 2026-07
    95/100 stars

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    Efficiency of transduction with the rAAV/DJ vector of the A375, G-361, SK-MEL-1, and CCD-1064Sk cells under hyperthermia. The results are shown for non-transduced cells (control) and transduced cells (rAAV/DJ) under standard conditions (37 °C) and underhyperthermia (43 °C). The results are presented as a mean of two independent experiments conducted in three repeats ± SD. The number of GFP+ (green fluorescent protein positive) cells was measured with an automatic cell counter ( A ) and a flow cytometer (FACS) ( B ). ( C ) The number of rAAV/DJ vector genome copies was measured in the analyzed cells with the qPCR method. Statistically significant differences between 37 °C and 43 °C are marked with asterisks (*** p < 0.001; **** p < 0.0001). ( D ) Cell viability after exposure to the hyperthermia condition. The results are presented as a percentage of live cells. The data were measured with FACS. Statistically significant differences between the number of live cells at 37 °C (control) and values in other conditions are marked with asterisks (* p < 0.05; ** p < 0.01).

    Journal: Current Issues in Molecular Biology

    Article Title: Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells

    doi: 10.3390/cimb45100537

    Figure Lengend Snippet: Efficiency of transduction with the rAAV/DJ vector of the A375, G-361, SK-MEL-1, and CCD-1064Sk cells under hyperthermia. The results are shown for non-transduced cells (control) and transduced cells (rAAV/DJ) under standard conditions (37 °C) and underhyperthermia (43 °C). The results are presented as a mean of two independent experiments conducted in three repeats ± SD. The number of GFP+ (green fluorescent protein positive) cells was measured with an automatic cell counter ( A ) and a flow cytometer (FACS) ( B ). ( C ) The number of rAAV/DJ vector genome copies was measured in the analyzed cells with the qPCR method. Statistically significant differences between 37 °C and 43 °C are marked with asterisks (*** p < 0.001; **** p < 0.0001). ( D ) Cell viability after exposure to the hyperthermia condition. The results are presented as a percentage of live cells. The data were measured with FACS. Statistically significant differences between the number of live cells at 37 °C (control) and values in other conditions are marked with asterisks (* p < 0.05; ** p < 0.01).

    Article Snippet: For cell transduction under hyperthermia conditions, the rAAV/DJ/CAG/GFP mosaic vector (rAAV/DJ; Cat. No. 7078; Vector Biolabs, Malvern, PA, USA) was used at the multiplicity of infection (MOI) of 4 × 10 4 gene copies.

    Techniques: Transduction, Plasmid Preparation, Flow Cytometry

    Efficiency of transduction with the rAAV/DJ vector of the melanoma A375 and G-361 cells under hyperthermia. Representative dot plots and histograms present the number of live and dead cells (%) and the number of GFP+ cells (%) for non-transduced cells (control) and transduced cells (rAAV/DJ) under standard conditions (37 °C) and under hyperthermia (43 °C). The percentage of GFP+ cells was measured in the population of live cells.

    Journal: Current Issues in Molecular Biology

    Article Title: Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells

    doi: 10.3390/cimb45100537

    Figure Lengend Snippet: Efficiency of transduction with the rAAV/DJ vector of the melanoma A375 and G-361 cells under hyperthermia. Representative dot plots and histograms present the number of live and dead cells (%) and the number of GFP+ cells (%) for non-transduced cells (control) and transduced cells (rAAV/DJ) under standard conditions (37 °C) and under hyperthermia (43 °C). The percentage of GFP+ cells was measured in the population of live cells.

    Article Snippet: For cell transduction under hyperthermia conditions, the rAAV/DJ/CAG/GFP mosaic vector (rAAV/DJ; Cat. No. 7078; Vector Biolabs, Malvern, PA, USA) was used at the multiplicity of infection (MOI) of 4 × 10 4 gene copies.

    Techniques: Transduction, Plasmid Preparation

    Efficiency of transduction with the rAAV/DJ vector of the SK-MEL-1 melanoma cells and normal fibroblasts CCD-1064Sk under hyperthermia. Representative dot plots and histograms present the number of live and dead cells (%) and the number of GFP+ cells (%) for non-transduced cells (control) and transduced cells (rAAV/DJ) under standard conditions (37 °C) and under hyperthermia (43 °C). The percentage of GFP+ cells was measured in the population of live cells.

    Journal: Current Issues in Molecular Biology

    Article Title: Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells

    doi: 10.3390/cimb45100537

    Figure Lengend Snippet: Efficiency of transduction with the rAAV/DJ vector of the SK-MEL-1 melanoma cells and normal fibroblasts CCD-1064Sk under hyperthermia. Representative dot plots and histograms present the number of live and dead cells (%) and the number of GFP+ cells (%) for non-transduced cells (control) and transduced cells (rAAV/DJ) under standard conditions (37 °C) and under hyperthermia (43 °C). The percentage of GFP+ cells was measured in the population of live cells.

    Article Snippet: For cell transduction under hyperthermia conditions, the rAAV/DJ/CAG/GFP mosaic vector (rAAV/DJ; Cat. No. 7078; Vector Biolabs, Malvern, PA, USA) was used at the multiplicity of infection (MOI) of 4 × 10 4 gene copies.

    Techniques: Transduction, Plasmid Preparation